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ICC/IF analysis of SH-SY-5Y cells using GTX11307 beta Tubulin antibody [D66] at 0.5 μg/mL(red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.
ICC/IF analysis of SH-SY-5Y cells using GTX11307 beta Tubulin antibody [D66] at 0.5 μg/mL(red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.
ICC/IF analysis of SH-SY-5Y cells using GTX11307 beta Tubulin antibody [D66] at 0.5 μg/mL(red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

beta Tubulin antibody [D66]

GTX11307
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityAlgae, Bovine, Canine, Chicken, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sea Urchin, Other Species
TargetTUBB
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Overview

  • Supplier
    GeneTex
  • Product Name
    beta Tubulin antibody [D66]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5-1.0 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    D66
  • Concentration
    ~1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID203068
  • Target name
    TUBB
  • Target description
    tubulin beta class I
  • Target synonyms
    CDCBM6, CSCSC1, M40, OK/SW-cl.56, TUBB1, TUBB5, tubulin beta chain, beta Ib tubulin, epididymis secretory sperm binding protein, tubulin beta-1 chain, tubulin beta-5 chain, tubulin, beta polypeptide
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP07437
  • Protein Name
    Tubulin beta chain
  • Scientific Description
    Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.
  • Reactivity
    Algae, Bovine, Canine, Chicken, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sea Urchin, Other Species
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Lee WJ, Chen LC, Lin JH, et al. Melatonin promotes neuroblastoma cell differentiation by activating hyaluronan synthase 3-induced mitophagy. Cancer Med. 2019,8(10):4821-4835. doi: 10.1002/cam4.2389
    Read this paper
  • Lin JH, Lee WJ, Wu HC, et al. Small G protein signalling modulator 2 (SGSM2) is involved in oestrogen receptor-positive breast cancer metastasis through enhancement of migratory cell adhesion via interaction with E-cadherin. Cell Adh Migr. 2019,13(1):120-137. doi: 10.1080/19336918.2019.1568139
    Read this paper
  • Juenemann K, Weisse C, Reichmann D, et al. Modulation of mutant huntingtin N-terminal cleavage and its effect on aggregation and cell death. Neurotox Res. 2011,20(2):120-33. doi: 10.1007/s12640-010-9227-6
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Beta Tubulin using anti-Beta Tubulin antibody (A01857-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: chicken heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta Tubulin antigen affinity purified polyclonal antibody (Catalog # A01857-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Beta Tubulin at approximately 50 kDa. The expected band size for Beta Tubulin is at 50 kDa.
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