![ICC/IF analysis of HeLa cells using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:5000 (green) with DAPI(blue).Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100. ICC/IF analysis of HeLa cells using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:5000 (green) with DAPI(blue).Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.](https://www.genetex.com/upload/website/prouct_img/normal/GTX11312/GTX11312_20170605_ICCIF_w_23060500_752.webp)
ICC/IF analysis of HeLa cells using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:5000 (green) with DAPI(blue).Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.
beta Tubulin I antibody [SAP.4G5]
GTX11312
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTUBB
Overview
- SupplierGeneTex
- Product Namebeta Tubulin I antibody [SAP.4G5]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:20,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDSAP.4G5
- ConjugateUnconjugated
- Gene ID203068
- Target nameTUBB
- Target descriptiontubulin beta class I
- Target synonymsCDCBM6, CSCSC1, M40, OK/SW-cl.56, TUBB1, TUBB5, tubulin beta chain, beta Ib tubulin, epididymis secretory sperm binding protein, tubulin beta-1 chain, tubulin beta-5 chain, tubulin, beta polypeptide
- HostMouse
- IsotypeIgG1
- Protein IDP07437
- Protein NameTubulin beta chain
- Scientific DescriptionThe major building block of microtubules is tubulin. Tubulin exists as a heterodimer of two similar but not identical polypeptides, designated alpha and beta, that assembles into microtubules. For beta-tubulin, six evolutionarily conserved isotypes were identified (designated beta I - beta VI). The most complex pattern of isotype distribution in tissues is seen in the vertebrate beta-tubulins. In mammals and birds, betaI is constitutive and found in most tissues.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![WB analysis of (1) chicken fibroblasts (2) mouse brain extract I (3) mouse brain extract II (4) rat brain (5) Jurkat (6) Daudi (7) SK-Mel-28 (8) G361 (9) HeLa using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:20000. WB analysis of (1) chicken fibroblasts (2) mouse brain extract I (3) mouse brain extract II (4) rat brain (5) Jurkat (6) Daudi (7) SK-Mel-28 (8) G361 (9) HeLa using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:20000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX11312/GTX11312_20170605_WB_w_23060500_398.webp)

![ICC/IF analysis of 3T3 cells using GTX27792 beta Tubulin antibody [TU-06]. Green : Primary antibody Blue : DAPI](https://www.genetex.com/upload/website/prouct_img/normal/GTX27792/GTX27792_20191025_AP_002_196_w_23060722_713.webp)
![ICC/IF analysis of SH-SY-5Y cells using GTX11307 beta Tubulin antibody [D66] at 0.5 microg/mL(red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.](https://www.genetex.com/upload/website/prouct_img/normal/GTX11307/GTX11307_20170605_ICCIF_w_23060500_266.webp)
![WB analysis of chicken fibroblasts extract using GTX11311 beta Tubulin antibody [2-28-33]. Lane A : Antibody dilution 1:200 Lane B : Negative control (only secondary antibody)](https://www.genetex.com/upload/website/prouct_img/normal/GTX11311/GTX11311_20170605_WB_w_23060500_274.webp)