ICC/IF analysis of HeLa cells using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:5000 (green) with DAPI(blue).Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.
![WB analysis of (1) chicken fibroblasts (2) mouse brain extract I (3) mouse brain extract II (4) rat brain (5) Jurkat (6) Daudi (7) SK-Mel-28 (8) G361 (9) HeLa using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:20000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX11312/GTX11312_20170605_WB_w_23060500_398.webp "WB analysis of (1) chicken fibroblasts (2) mouse brain extract I (3) mouse brain extract II (4) rat brain (5) Jurkat (6) Daudi (7) SK-Mel-28 (8) G361 (9) HeLa using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:20000.")
ICC/IF analysis of HeLa cells using GTX11312 beta Tubulin I antibody [SAP.4G5] at 1:5000 (green) with DAPI(blue).Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.
beta Tubulin I antibody [SAP.4G5]
GTX11312
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Hamster, Human, Mouse, Rat, Xenopus
TargetTUBB
Overview
- SupplierGeneTex
- Product Namebeta Tubulin I antibody [SAP.4G5]
- Delivery Days Customer9
- Antibody SpecificityRecognizes an epitope located in the C-terminal sequence of β-tubulin isotype I. No reactivity with other tubulin isotypes is observed.
- Application Supplier NoteWB: 1:20,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDSAP.4G5
- ConjugateUnconjugated
- Gene ID203068
- Target nameTUBB
- Target descriptiontubulin beta class I
- Target synonymsbeta Ib tubulin; CDCBM6; CSCSC1; epididymis secretory sperm binding protein; M40; OK/SW-cl.56; TUBB1; TUBB5; tubulin beta chain; tubulin beta-1 chain; tubulin beta-5 chain; tubulin, beta polypeptide
- HostMouse
- IsotypeIgG1
- Protein IDP07437
- Protein NameTubulin beta chain
- Scientific DescriptionThe major building block of microtubules is tubulin. Tubulin exists as a heterodimer of two similar but not identical polypeptides, designated alpha and beta, that assembles into microtubules. For beta-tubulin, six evolutionarily conserved isotypes were identified (designated beta I - beta VI). The most complex pattern of isotype distribution in tissues is seen in the vertebrate beta-tubulins. In mammals and birds, betaI is constitutive and found in most tissues.
- ReactivityBovine, Canine, Chicken, Hamster, Human, Mouse, Rat, Xenopus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203