More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/beta Tubulin I antibody [SAP.4G5]

beta Tubulin I antibody [SAP.4G5]

GTX11312
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Hamster, Human, Mouse, Rat, Xenopus
TargetTUBB
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    beta Tubulin I antibody [SAP.4G5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:20,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SAP.4G5
  • Conjugate
    Unconjugated
  • Gene ID203068
  • Target name
    TUBB
  • Target description
    tubulin beta class I
  • Target synonyms
    CDCBM6, CSCSC1, M40, OK/SW-cl.56, TUBB1, TUBB5, tubulin beta chain, beta Ib tubulin, epididymis secretory sperm binding protein, tubulin beta-1 chain, tubulin beta-5 chain, tubulin, beta polypeptide
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP07437
  • Protein Name
    Tubulin beta chain
  • Scientific Description
    The major building block of microtubules is tubulin. Tubulin exists as a heterodimer of two similar but not identical polypeptides, designated alpha and beta, that assembles into microtubules. For beta-tubulin, six evolutionarily conserved isotypes were identified (designated beta I - beta VI). The most complex pattern of isotype distribution in tissues is seen in the vertebrate beta-tubulins. In mammals and birds, betaI is constitutive and found in most tissues.
  • Reactivity
    Bovine, Canine, Chicken, Hamster, Human, Mouse, Rat, Xenopus
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Gumuscu B, Herr AE. Separation-encoded microparticles for single-cell western blotting. Lab Chip. 2020,20(1):64-73. doi: 10.1039/c9lc00917e
    Read this paper
  • Jung D, Khurana A, Roy D, et al. Quinacrine upregulates p21/p27 independent of p53 through autophagy-mediated downregulation of p62-Skp2 axis in ovarian cancer. Sci Rep. 2018,8(1):2487. doi: 10.1038/s41598-018-20531-w
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-Beta Tubulin type VI [AA16 (6A10)]Ab02684-10.0
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mammals
TargetTUBB
  • SizePrice
Product group Antibodies
Biosynth
beta Tubulin antibody70R-13069
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Beta-tubulin (166-183aa) Antibody130-10977
ApplicationsELISA
ReactivityHuman
TargetTUBB
  • SizePrice
Figure 1. Western blot analysis of Beta Tubulin using anti-Beta Tubulin antibody (A01857-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: chicken heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta Tubulin antigen affinity purified polyclonal antibody (Catalog # A01857-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Beta Tubulin at approximately 50 kDa. The expected band size for Beta Tubulin is at 50 kDa.
Product group Antibodies
Boster Bio
Anti-Beta Tubulin/TUBB Antibody Picoband(r)A01857-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityChicken, Human, Monkey, Mouse, Rat
TargetTUBB
  • SizePrice
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta Tubulin antibody (GTX101279) at 1:300 dilution.
Product group Antibodies
References
GeneTex
beta Tubulin antibodyGTX101279
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHamster, Human, Mouse, Rat, Zebra Fish
TargetTUBB
  • SizePrice
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using beta Tubulin(GTX112658) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Product group Antibodies
References
GeneTex
beta Tubulin antibodyGTX112658
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetTUBB
  • SizePrice
Immunofluorescence analysis of methanol-fixed HeLa, using beta Tubulin(GTX112659) antibody at 1:500 dilution.
Product group Antibodies
GeneTex
beta Tubulin antibodyGTX112659
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetTUBB
  • SizePrice
ICC/IF analysis of SH-SY-5Y cells using GTX11307 beta Tubulin antibody [D66] at 0.5 μg/mL(red) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.
Product group Antibodies
References
GeneTex
beta Tubulin antibody [D66]GTX11307
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityAlgae, Bovine, Canine, Chicken, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Sea Urchin, Other Species
TargetTUBB
  • SizePrice
Product group Antibodies
GeneTex
beta Tubulin antibody [2-28-33]GTX11311
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityBovine, C. Elegans, Chicken, Human, Rat, Sea Urchin, Other Species
TargetTUBB
  • SizePrice
WB analysis of pig brain tissue lysate using GTX79865 beta Tubulin antibody [TU-13].
Product group Antibodies
GeneTex
beta Tubulin antibody [TU-13]GTX79865
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Plant, Porcine
TargetTUBB
  • SizePrice