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BF1 / FOXG1 Antibody

LS-C832662
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetFOXG1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BF1 / FOXG1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:2000 - 1:5000), IHC (1:45 - 1:300), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.08 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2290
  • Target name
    FOXG1
  • Target description
    forkhead box G1
  • Target synonyms
    BF1; BF2; brain factor 1; brain factor 2; FHKL3; FKH2; FKHL1; FKHL2; FKHL3; FKHL4; forkhead box protein G1; forkhead-like 1; forkhead-like 2; forkhead-like 3; forkhead-like 4; FOXG1A; FOXG1B; FOXG1C; HBF-1; HBF2; HBF-2; HBF-3; HBF-G2; HFK1; HFK2; HFK3; KHL2; oncogene QIN; QIN
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXG1 using anti-FOXG1 antibody (A01852-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXG1 antigen affinity purified polyclonal antibody (Catalog # A01852-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXG1 at approximately 52 kDa. The expected band size for FOXG1 is at 52 kDa.
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