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ICC/IF analysis of U2OS cells using GTX03427 FOXG1 antibody. Blue : DAPI for nuclear staining Dilution : 1:100
ICC/IF analysis of U2OS cells using GTX03427 FOXG1 antibody. Blue : DAPI for nuclear staining Dilution : 1:100
ICC/IF analysis of U2OS cells using GTX03427 FOXG1 antibody. Blue : DAPI for nuclear staining Dilution : 1:100

FOXG1 antibody

GTX03427
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetFOXG1
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Overview

  • Supplier
    GeneTex
  • Product Name
    FOXG1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2290
  • Target name
    FOXG1
  • Target description
    forkhead box G1
  • Target synonyms
    BF1, BF2, FHKL3, FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, HBF-1, HBF-2, HBF-3, HBF-G2, HBF2, HFK1, HFK2, HFK3, KHL2, QIN, forkhead box protein G1, brain factor 1, brain factor 2, forkhead-like 1, forkhead-like 2, forkhead-like 3, forkhead-like 4, oncogene QIN
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55316
  • Protein Name
    Forkhead box protein G1
  • Scientific Description
    This locus encodes a member of the forked-head transcription factor family. The encoded protein, which functions as a repressor, may play a role in brain development. Mutations at this locus have been associated with Rett syndrome. [provided by RefSeq, Feb 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXG1 using anti-FOXG1 antibody (A01852-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXG1 antigen affinity purified polyclonal antibody (Catalog # A01852-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXG1 at approximately 52 kDa. The expected band size for FOXG1 is at 52 kDa.
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