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BHMT Antibody (YA2549, Rabbit)

HY-P82804
MedChem Express
TargetBHMT
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    BHMT Antibody (YA2549, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID635
  • Target name
    BHMT
  • Target description
    betaine--homocysteine S-methyltransferase
  • Target synonyms
    BHMT1, HEL-S-61p, betaine--homocysteine S-methyltransferase 1, epididymis secretory sperm binding protein Li 61p, phi-crystallin
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    BHMT Antibody (YA2549) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2549), targeting BHMT, with a predicted molecular weight of 45 kDa (observed band size: 45 kDa). BHMT Antibody (YA2549) can be used for WB, IHC-P, IP experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BHMT using anti-BHMT antibody (A06712-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BHMT antigen affinity purified polyclonal antibody (Catalog # A06712-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BHMT at approximately 45KD. The expected band size for BHMT is at 45KD.
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