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Goat anti-BHMT

EB07943
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBHMT
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-BHMT Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID635
  • Target name
    BHMT
  • Target description
    betaine--homocysteine S-methyltransferase
  • Target synonyms
    betaine--homocysteine S-methyltransferase 1; BHMT1; epididymis secretory sperm binding protein Li 61p; HEL-S-61p
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001704.1. GeneIDs all Nonhuman: 12116 (mouse); 81508 (rat);. Purification: Antigen affinity purified. Names and symbols: BHMT; betaine-homocysteine methyltransferase
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Alleviation of paraquat-induced oxidative lung injury by betaine via regulation of sulfur-containing amino acid metabolism despite the lack of betaine-homocysteine methyltransferase (BHMT) in the lung. Na JD et al., 2019 Feb 20, Food Funct
    Read more
  • Age-Related Changes in Sulfur Amino Acid Metabolism in Male C57BL/6 Mice. Jeon JS et al., 2018 Mar 1, Biomol Ther (Seoul)
    Read more
  • Alleviation of hepatic fat accumulation by betaine involves reduction of homocysteine via up-regulation of betaine-homocysteine methyltransferase (BHMT). Ahn CW et al., 2016 Aug 26, Biochem Biophys Res Commun
    Read more
  • Alterations in the metabolomics of sulfur-containing substances in rat kidney by betaine. Kim YC et al., 2014 Apr, Amino Acids
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  • Alterations in sulfur amino acid metabolism in mice treated with silymarin: a novel mechanism of its action involved in enhancement of the antioxidant defense in liver. Kwon DY et al., 2013 Aug, Planta Med
    Read more
  • Hepatic metabolism of sulfur amino acids in db/db mice. Yun KU et al., 2013 Mar, Food Chem Toxicol
    Read more
  • Plasma homocysteine level and hepatic sulfur amino acid metabolism in mice fed a high-fat diet. Yun KU et al., 2013 Feb, Eur J Nutr
    Read more
  • Sulfur amino acid metabolism in doxorubicin-resistant breast cancer cells. Ryu CS et al., 2011 Aug 15, Toxicol Appl Pharmacol
    Read more

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Figure 1. Western blot analysis of BHMT using anti-BHMT antibody (A06712-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BHMT antigen affinity purified polyclonal antibody (Catalog # A06712-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BHMT at approximately 45KD. The expected band size for BHMT is at 45KD.
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