Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 12% SDS PAGE GTX101323 diluted at 1:1000
![Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3] (GTX101323) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101323/GTX101323_39897_20180824_WB_shRNA_watermark_w_23060100_634.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3] (GTX101323) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Bid antibody [N1C3] immunoprecipitates BID protein in IP experiments. IP samples: Jurkat whole cell extract A. 40 μg Jurkat whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of BID protein by 4 μg Bid antibody [N1C3] (GTX101323) 5 % SDS-PAGE The immunoprecipitated BID protein was detected by Bid antibody [N1C3] (GTX101323) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX101323/GTX101323_39897_IP_w_23060100_279.webp "Bid antibody [N1C3] immunoprecipitates BID protein in IP experiments. IP samples: Jurkat whole cell extract A. 40 μg Jurkat whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of BID protein by 4 μg Bid antibody [N1C3] (GTX101323) 5 % SDS-PAGE The immunoprecipitated BID protein was detected by Bid antibody [N1C3] (GTX101323) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
![Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3] (GTX101323) or Bid antibody [N1C3-2] (GTX110568). Western blot analysis was performed using Bid antibody [N1C3] (GTX101323) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101323/GTX101323_39897_IP_2_w_23060100_791.webp "Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3] (GTX101323) or Bid antibody [N1C3-2] (GTX110568). Western blot analysis was performed using Bid antibody [N1C3] (GTX101323) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 12% SDS PAGE GTX101323 diluted at 1:1000
Bid antibody [N1C3]
GTX101323
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetBID
Overview
- SupplierGeneTex
- Product NameBid antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.8 mg/ml
- ConjugateUnconjugated
- Gene ID637
- Target nameBID
- Target descriptionBH3 interacting domain death agonist
- Target synonymsFP497, BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist, desmocollin type 4, p22 BID
- HostRabbit
- IsotypeIgG
- Protein IDP55957
- Protein NameBH3-interacting domain death agonist
- Scientific DescriptionThis gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8Read this paper
Datasheet
Related products
Product group Antibodies
Anti-BID Antibody, Biotinylated130-10027B-50
ApplicationsELISA
ReactivityHuman
TargetBID
- SizePrice
![Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] (GTX110568) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110568/GTX110568_40051_20180824_WB_shRNA_watermark_w_23060500_778.webp)
Product group Antibodies
References
Bid antibody [N1C3-2]GTX110568
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetBID
- SizePrice
Product group Antibodies
Bid antibody [4D3]GTX57542
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetBID
- SizePrice
![ICC/IF analysis of HeLa cells using GTX60429 Bid antibody [3C5]. Green : Bid Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX60429/GTX60429_20170912_ICCIF_w_23061123_825.webp)
Product group Antibodies
References
Bid antibody [3C5]GTX60429
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetBID
- SizePrice
Product group Antibodies
Bid Polyclonal AntibodyCAC11767
ApplicationsELISA, ImmunoHistoChemistry
TargetBID
- SizePrice
Product group Antibodies
Bid Recombinant Antibody, AbBy Fluor-488 ConjugatedBSM-61450R-BF488
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetBID
- SizePrice
Product group Antibodies
Bid antibody, C-termGTX89562
ApplicationsWestern Blot
ReactivityHuman
TargetBID
- SizePrice
Product group Antibodies
Anti-BID AntibodyA98541
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
- SizePrice