WB analysis of Jurkat cells without added BID (Lanes 1 and 2), with caspase-8 cleaved recombinant human BID (Lane 3), or with caspase-8 cleaved recombinant mouse BID (Lane 4) using either a human BID full length antibody (lane 1) or GTX25498 Bid (p15-specific ) antibody (lanes 2-4). The data show that the human BID (p15) antibody recognizes only the human 15 kDa BID fragment, demonstrating the specificity of the antibody.
WB analysis of Jurkat cells without added BID (Lanes 1 and 2), with caspase-8 cleaved recombinant human BID (Lane 3), or with caspase-8 cleaved recombinant mouse BID (Lane 4) using either a human BID full length antibody (lane 1) or GTX25498 Bid (p15-specific ) antibody (lanes 2-4). The data show that the human BID (p15) antibody recognizes only the human 15 kDa BID fragment, demonstrating the specificity of the antibody.
Bid (p15-specific ) antibody
GTX25498
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBID
Overview
- SupplierGeneTex
- Product NameBid (p15-specific ) antibody
- Delivery Days Customer9
- Antibody SpecificityRecognizes the human C-terminal 15 kDa fragment of BID (p15).
- Application Supplier NoteICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID637
- Target nameBID
- Target descriptionBH3 interacting domain death agonist
- Target synonymsapoptic death agonist; BH3-interacting domain death agonist; desmocollin type 4; FP497; Human BID coding sequence; p22 BID
- HostRabbit
- IsotypeIgG
- Protein IDP55957
- Protein NameBH3-interacting domain death agonist
- Scientific DescriptionThis gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Alternative Fas-mediated cell death pathway is dependent on the different cleavage patterns of procaspase-8. Li Y et al., 2009 Nov, Mol Cell BiochemRead more