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ICC/IF analysis of human endothelial cells using GTX57229 Uteroglobin antibody. Dilution : 1:50 Fixation: 4% PFA
ICC/IF analysis of human endothelial cells using GTX57229 Uteroglobin antibody. Dilution : 1:50 Fixation: 4% PFA
ICC/IF analysis of human endothelial cells using GTX57229 Uteroglobin antibody. Dilution : 1:50 Fixation: 4% PFA

Biglycan antibody

GTX57229
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetBGN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Biglycan antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:300-1:1000. ICC/IF: 1:50-1:200. IHC-P: 1:200-1:400. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID633
  • Target name
    BGN
  • Target description
    biglycan
  • Target synonyms
    biglycan; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; DSPG1; MRLS; PGI; PG-S1; SEMDX; SLRR1A; small leucine-rich protein 1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21810
  • Protein Name
    Biglycan
  • Scientific Description
    This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in bone growth, muscle development and regeneration, and collagen fibril assembly in multiple tissues. This protein may also regulate inflammation and innate immunity. Additionally, the encoded protein may contribute to atherosclerosis and aortic valve stenosis in human patients. This gene and the related gene decorin are thought to be the result of a gene duplication. [provided by RefSeq, Nov 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Biglycan/BGN using anti-Biglycan/BGN antibody (A02090-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO320 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HUVEC whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat L6 whole cell lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Biglycan/BGN antigen affinity purified polyclonal antibody (Catalog # A02090-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Biglycan/BGN at approximately 48 kDa. The expected band size for Biglycan/BGN is at 42 kDa.
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