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Biotin Anti-Mouse CD120b Antibody

RD20546F
Reddot Biotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetTnfrsf1b
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    Biotin Anti-Mouse CD120b Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TR75-54.7
  • Concentration
    0.5 mg/ml
  • Conjugate
    Biotin
  • Gene ID21938
  • Target name
    Tnfrsf1b
  • Target description
    tumor necrosis factor receptor superfamily, member 1b
  • Target synonyms
    CD120b; p7; p75; p75 TNFR; p80 TNF-alpha receptor; TN; Tnf; TNF-; TNF receptor beta chain; TNF-a; TNFal; TNF-alphaR2; TNFalpha-R2; TNFBR; TNFR; TNF-R; Tnfr-1; Tnfr2; TNF-R2; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor type II
  • Host
    Hamster
  • Isotype
    IgG
  • Scientific Description
    CD120b is a 75 kD type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type II (TNFRII) or p75. It is expressed on a variety of cells at low levels; the expression is upregulated upon activation. This receptor binds both TNF-Oe+/- and LT-Oe+/- (also known as TNF-beta). In association with TRAF1 and TRAF2, the receptor crosslinking induced by TNF-Oe+/- or LT-Oe+/- trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF Receptor II using anti-TNF Receptor II antibody (PB9515). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Mouse Thymus Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor II antigen affinity purified polyclonal antibody (Catalog # PB9515) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor II at approximately 75 kDa. The expected band size for TNF Receptor II is at 75 kDa.
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