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Goat anti-Survivin / BIRC5 Antibody

ORB18952
Biorbyt
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetBIRC5
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Goat anti-Survivin / BIRC5 Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 18kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 16.4kDa according to NP_001159.2 and 18.6kDa according to NP_001012271.1). Recommended concentration: 0.01-0.5 microg/ml.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA: 1:16000, WB: 0.01-0.5 μg/ml ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID332
  • Target name
    BIRC5
  • Target description
    baculoviral IAP repeat containing 5
  • Target synonyms
    API4, EPR-1, baculoviral IAP repeat-containing protein 5, apoptosis inhibitor 4, apoptosis inhibitor survivin
  • Host
    Goat
  • Scientific Description
    Goat polyclonal antibody to BIRC5
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (A00379). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
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