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ProductsBack/BIRC5 Mouse Monoclonal Antibody [Clone ID: OTI2D9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BIRC5 (Cat# RC205935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC5 (Cat# TA501245). Positive lysates [LY400469] (100ug) and [LC400469] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BIRC5 (Cat# RC205935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC5 (Cat# TA501245). Positive lysates [LY400469] (100ug) and [LC400469] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BIRC5 (Cat# RC205935, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC5 (Cat# TA501245). Positive lysates [LY400469] (100ug) and [LC400469] (20ug) can be purchased separately from OriGene.

BIRC5 Mouse Monoclonal Antibody [Clone ID: OTI2D9]

CF501245
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBIRC5
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    BIRC5 Mouse Monoclonal Antibody [Clone ID: OTI2D9]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2D9
  • Gene ID332
  • Target name
    BIRC5
  • Target description
    baculoviral IAP repeat containing 5
  • Target synonyms
    API4, EPR-1, baculoviral IAP repeat-containing protein 5, apoptosis inhibitor 4, apoptosis inhibitor survivin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO15392
  • Protein Name
    Baculoviral IAP repeat-containing protein 5
  • Scientific Description
    Carrier-free (BSA/glycerol-free) BIRC5/Survivin mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (A00379). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
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