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Detection of survivin in HeLa whole cell lysate (30 ug)
Detection of survivin in HeLa whole cell lysate (30 ug)
Detection of survivin in HeLa whole cell lysate (30 ug)

BIRC5 Rabbit Polyclonal Antibody

TA301775
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Feline, Human, Mouse, Rat
TargetBIRC5
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    BIRC5 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID332
  • Target name
    BIRC5
  • Target description
    baculoviral IAP repeat containing 5
  • Target synonyms
    API4, EPR-1, baculoviral IAP repeat-containing protein 5, apoptosis inhibitor 4, apoptosis inhibitor survivin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15392
  • Protein Name
    Baculoviral IAP repeat-containing protein 5
  • Scientific Description
    Rabbit Polyclonal Antibody against Survivin - Astrocyte Marker
  • Reactivity
    Canine, Feline, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (A00379). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
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