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BIRC5 / Survivin Antibody (AF488)

LS-C39342
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBIRC5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BIRC5 / Survivin Antibody (AF488)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Suitable for use in Immunofluorescence, Immunocytochemistry and Flow Cytometry. Immunofluorescence (IF-IC): 1:10. Add 10 ul to 5x10^5 cells in 90 ul PBS/0,5% BSA. Flow Cytometry: 1:10. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. Flo (1:10), ICC, IF (1:10) Suitable for use in Immunofluorescence, Immunocytochemistry and Flow Cytometry. Immunofluorescence (IF-IC): 1:10. Add 10 ul to 5x10^5 cells in 90 ul PBS/0,5% BSA. Flow Cytometry: 1:10. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    AlexaFluor 488
  • Estimated Purity
    ...
  • Gene ID332
  • Target name
    BIRC5
  • Target description
    baculoviral IAP repeat containing 5
  • Target synonyms
    API4, EPR-1, baculoviral IAP repeat-containing protein 5, apoptosis inhibitor 4, apoptosis inhibitor survivin
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (A00379). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
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