BIRC5 / Survivin Antibody

LifeSpan BioSciences

BIRC5 / Survivin Antibody

23

Western blot analysis of Survivin expression in K562 (A) whole cell lysates. Immunofluorescent analysis of Survivin staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.. ICC, IF (1:50 - 1:200), WB (1:500 - 1:2000) Western blot analysis of Survivin expression in K562 (A) whole cell lysates. Immunofluorescent analysis of Survivin staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

ImmunoFluorescence, Western Blot, ImmunoCytoChemistry

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

...

BIRC5

baculoviral IAP repeat containing 5

API4, EPR-1, baculoviral IAP repeat-containing protein 5, apoptosis inhibitor 4, apoptosis inhibitor survivin

Rabbit

Human

-20°C

12352203