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ProductsBack/BIRC5 / Survivin Antibody (Preservative Free, Biotin)

BIRC5 / Survivin Antibody (Preservative Free, Biotin)

LS-C343484
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetBIRC5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BIRC5 / Survivin Antibody (Preservative Free, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    This antibody is shipped with antibody diluent; we recommend the addition of 100 ul of diluent to the 50 ug of lyophilized antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. ELISA (1:40000) This antibody is shipped with antibody diluent; we recommend the addition of 100 ul of diluent to the 50 ug of lyophilized antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID332
  • Target name
    BIRC5
  • Target description
    baculoviral IAP repeat containing 5
  • Target synonyms
    API4, EPR-1, baculoviral IAP repeat-containing protein 5, apoptosis inhibitor 4, apoptosis inhibitor survivin
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (A00379). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
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