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Western Blot analysis of 293T cells using Blk Polyclonal Antibody
Western Blot analysis of 293T cells using Blk Polyclonal Antibody
Western Blot analysis of 293T cells using Blk Polyclonal Antibody

BLK Antibody

CSB-PA000997
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBLK
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Overview

  • Supplier
    Cusabio
  • Product Name
    BLK Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID640
  • Target name
    BLK
  • Target description
    BLK proto-oncogene, Src family tyrosine kinase
  • Target synonyms
    b lymphocyte kinase; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase; MODY11; p55-Blk; tyrosine-protein kinase Blk
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51451
  • Protein Name
    Tyrosine-protein kinase Blk
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BLK using anti-BLK antibody (A01539-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BLK antigen affinity purified polyclonal antibody (Catalog # A01539-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BLK at approximately 60 kDa. The expected band size for BLK is at 60 kDa.
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