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Western Blot analysis of 293 cells using BLNK Polyclonal Antibody
Western Blot analysis of 293 cells using BLNK Polyclonal Antibody
Western Blot analysis of 293 cells using BLNK Polyclonal Antibody

BLNK Antibody

CSB-PA000998
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetBLNK
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Overview

  • Supplier
    Cusabio
  • Product Name
    BLNK Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29760
  • Target name
    BLNK
  • Target description
    B cell linker
  • Target synonyms
    AGM4; B cell adaptor containing SH2 domain; BASH; bca; B-cell activation; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; B-cell linker protein; BLNK-S; cytoplasmic adapter protein; LY57; SLP65; SLP-65; Src homology [SH2] domain-containing leukocyte protein of 65 kD; Src homology 2 domain-containing leukocyte protein of 65 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WV28
  • Protein Name
    B-cell linker protein
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BLNK using anti-BLNK antibody (A03630-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BLNK antigen affinity purified polyclonal antibody (Catalog # A03630-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BLNK at approximately 70 kDa. The expected band size for BLNK is at 70 kDa.
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