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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Phospho-BLNK(Tyr96)Polyclonal Antibody, Unconjugated (bs-3054R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Phospho-BLNK(Tyr96)Polyclonal Antibody, Unconjugated (bs-3054R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Phospho-BLNK(Tyr96)Polyclonal Antibody, Unconjugated (bs-3054R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Phospho-BLNK (Tyr96) Polyclonal Antibody

BS-3054R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBLNK
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Overview

  • Supplier
    Bioss
  • Product Name
    BLNK(Tyr96) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID29760
  • Target name
    BLNK
  • Target description
    B cell linker
  • Target synonyms
    AGM4, BASH, BLNK-S, LY57, SLP-65, SLP65, bca, B-cell linker protein, B cell adaptor containing SH2 domain, B-cell activation, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, Src homology [SH2] domain-containing leukocyte protein of 65 kD, cytoplasmic adapter protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WV28
  • Protein Name
    B-cell linker protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of BLNK using anti-BLNK antibody (A03630-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BLNK antigen affinity purified polyclonal antibody (Catalog # A03630-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BLNK at approximately 70 kDa. The expected band size for BLNK is at 70 kDa.
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