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Western Blot Positive WB detected in: K562 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate All lanes: BLVRB antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate All lanes: BLVRB antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate All lanes: BLVRB antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa

BLVRB Antibody

CSB-PA002722HA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBLVRB
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Overview

  • Supplier
    Cusabio
  • Product Name
    BLVRB Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID645
  • Target name
    BLVRB
  • Target description
    biliverdin reductase B
  • Target synonyms
    biliverdin-IX beta-reductase; BVRB; BVR-B; epididymis secretory protein Li 10; flavin reductase (NADPH); FLR; FR; GHBP; green heme-binding protein; HEL-S-10; NADPH-dependent diaphorase; NADPH-flavin reductase; SDR43U1; short chain dehydrogenase/reductase family 43U, member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30043
  • Protein Name
    Flavin reductase (NADPH)
  • Scientific Description
    Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BLVRB using anti-BLVRB antibody (A08072-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BLVRB antigen affinity purified polyclonal antibody (Catalog # A08072-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BLVRB at approximately 22 kDa. The expected band size for BLVRB is at 22-27 kDa.
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Immunohistochemical staining of human colon, liver, small intestine and spleen using Anti-BLVRB antibody HPA041698 (A) shows similar protein distribution across tissues to independent antibody HPA041937 (B).
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