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Bmp4 Polyclonal Antibody

CAC07069
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetBMP4
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Overview

  • Supplier
    Biomatik
  • Product Name
    BMP4 Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID652
  • Target name
    BMP4
  • Target description
    bone morphogenetic protein 4
  • Target synonyms
    BMP2B, BMP2B1, MCOPS6, OFC11, ZYME, bone morphogenetic protein 4, bone morphogenetic protein 2B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12644
  • Protein Name
    Bone morphogenetic protein 4
  • Scientific Description
    The Bmp4 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity Supplier
    Human, Mouse, Zebrafish
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BMP4 using anti-BMP4 antibody (A00321). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human BMP4 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP4 antigen affinity purified polyclonal antibody (Catalog # A00321) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP4 at approximately 22KD. The expected band size for BMP4 is at 13KD.
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