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BPIFA1 / SPLUNC1 Antibody (aa228-241)

LS-C186450
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetBPIFA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BPIFA1 / SPLUNC1 Antibody (aa228-241)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 26kD band observed in secretions of Human primary airway cells in culture-and in Human Bronchoalveolar Lavage fluid (calculated MW of 26.7kD according to NP_057667.1). Recommended concentration: 1-3 ug/ml.. Peptide-ELISA (1:64000), WB (1 - 3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 26kD band observed in secretions of Human primary airway cells in culture-and in Human Bronchoalveolar Lavage fluid (calculated MW of 26.7kD according to NP_057667.1). Recommended concentration: 1-3 ug/ml.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51297
  • Target name
    BPIFA1
  • Target description
    BPI fold containing family A member 1
  • Target synonyms
    bA49G10.5; BPI fold-containing family A member 1; ligand-binding protein RYA3; lung-specific protein X; LUNX; NASG; nasopharyngeal carcinoma-related protein; palate lung and nasal epithelium clone protein; palate, lung and nasal epithelium associated; PLUNC; protein Plunc; secretory protein in upper respiratory tracts; short PLUNC1; SPLUNC1; SPURT; tracheal epithelium enriched protein; von Ebner protein Hl
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Plunc/BPIFA1 using anti-Plunc/BPIFA1 antibody (A03162-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plunc/BPIFA1 antigen affinity purified polyclonal antibody (Catalog # A03162-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plunc/BPIFA1 at approximately 27 kDa. The expected band size for Plunc/BPIFA1 is at 27 kDa.
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