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BRCA1 antibody [8F7]

GTX70113
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetBRCA1
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Overview

  • Supplier
    GeneTex
  • Product Name
    BRCA1 antibody [8F7] - KO/KD Validated
  • Delivery Days Customer
    9
  • Antibody Specificity
    This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.
  • Application Supplier Note
    For IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. For IP: Use at an assay dependent dilution. For WB: 1:500-1:3000. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Optimal dilutions/concentrations should be determined by the researcher.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8F7
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID672
  • Target name
    BRCA1
  • Target description
    BRCA1 DNA repair associated
  • Target synonyms
    BRCA1/BRCA2-containing complex, subunit 1; BRCAI; BRCC1; breast and ovarian cancer susceptibility protein 1; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; BROVCA1; early onset breast cancer 1; Fanconi anemia, complementation group S; FANCS; IRIS; PNCA4; PPP1R53; protein phosphatase 1, regulatory subunit 53; PSCP; RING finger protein 53; RNF53
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP38398
  • Protein Name
    Breast cancer type 1 susceptibility protein
  • Scientific Description
    This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory.
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  • BRCA1-Dependent Translational Regulation in Breast Cancer Cells. Dacheux E et al., 2013, PLoS One
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  • Epigenetic modulation of BRCA1 and BRCA2 gene expression by equol in breast cancer cell lines. Bosviel R et al., 2012 Oct, Br J Nutr
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  • A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitor. Turner NC et al., 2008 May 7, EMBO J
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  • Epigenetic inactivation of the chromosomal stability control genes BRCA1, BRCA2, and XRCC5 in non-small cell lung cancer. Lee MN et al., 2007 Feb 1, Clin Cancer Res
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  • BRCA1 gene expression in breast cancer: a correlative study between real-time RT-PCR and immunohistochemistry. Al-Mulla F et al., 2005 May, J Histochem Cytochem
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  • Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia. Bernard-Gallon DJ et al., 2004 Jan-Feb, Anticancer Res
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  • BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest. Ongusaha PP et al., 2003 Jun 12, Oncogene
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  • The prognostic impact of hormone receptors and c-erbB-2 in pregnancy-associated breast cancer and their correlation with BRCA1 and cell cycle modulators. Reed W et al., 2003 Apr, Int J Surg Pathol
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  • Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization. Okada S et al., 2003 Jan 17, J Biol Chem
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