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ProductsBack/BRCA1-pS1524 Polyclonal Antibody AE00298
Confocal microscopy of HepG2 (LPS-treated for 4h) stained with BRCA1-pS1524 Rabbit Polyclonal Antibody AE00298 at 5ug/ml (1h at 37℃). Detection by AF594 (red) for the rabbit BRCA1-pS1524 antibody, AF488 (green) for the mouse beta-tubulin antibody and DAPI (blue) for nuclear staining.
Confocal microscopy of HepG2 (LPS-treated for 4h) stained with BRCA1-pS1524 Rabbit Polyclonal Antibody AE00298 at 5ug/ml (1h at 37℃). Detection by AF594 (red) for the rabbit BRCA1-pS1524 antibody, AF488 (green) for the mouse beta-tubulin antibody and DAPI (blue) for nuclear staining.
Confocal microscopy of HepG2 (LPS-treated for 4h) stained with BRCA1-pS1524 Rabbit Polyclonal Antibody AE00298 at 5ug/ml (1h at 37℃). Detection by AF594 (red) for the rabbit BRCA1-pS1524 antibody, AF488 (green) for the mouse beta-tubulin antibody and DAPI (blue) for nuclear staining.

BRCA1-pS1524 Polyclonal Antibody AE00298

AE00298
Aeonian Biotech
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetBRCA1
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    BRCA1-pS1524 Polyclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ICC, IHC, WB
  • Category Supplier
    Antibody
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID672
  • Target name
    BRCA1
  • Target description
    BRCA1 DNA repair associated
  • Target synonyms
    BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53, breast cancer type 1 susceptibility protein, BRCA1/BRCA2-containing complex, subunit 1, Fanconi anemia, complementation group S, RING finger protein 53, breast and ovarian cancer susceptibility protein 1, breast cancer 1, early onset, early onset breast cancer 1, protein phosphatase 1, regulatory subunit 53
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP38398
  • Protein Name
    Breast cancer type 1 susceptibility protein
  • Scientific Description
    BRCA1-pS1524 Polyclonal Antibody AE00298
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Human
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of BRCA1 using anti-BRCA1 antibody (A00005-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCA1 antigen affinity purified polyclonal antibody (Catalog # A00005-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCA1 at approximately 290 kDa. The expected band size for BRCA1 is at 210 kDa.
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