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BRCC36 Antibody (YA2298, Rabbit)

HY-P82553
MedChem Express
TargetBRCC3
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    BRCC36 Antibody (YA2298, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID79184
  • Target name
    BRCC3
  • Target description
    BRCA1/BRCA2-containing complex subunit 3
  • Target synonyms
    BRCA1/BRCA2-containing complex subunit 36; BRCA1-A complex subunit BRCC36; BRCC36; BRISC complex subunit BRCC36; C6.1A; CXorf53; lys-63-specific deubiquitinase BRCC36
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    BRCC36 Antibody (YA2298) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2298), targeting BRCC36, with a predicted molecular weight of 36 kDa (observed band size: 36 kDa). BRCC36 Antibody (YA2298) can be used for WB, IP experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BRCC36/BRCC3 using anti-BRCC36/BRCC3 antibody (A04690-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCC36/BRCC3 antigen affinity purified polyclonal antibody (Catalog # A04690-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCC36/BRCC3 at approximately 36 kDa. The expected band size for BRCC36/BRCC3 is at 36 kDa.
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