HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639557/GTX639557_T-45264_20240126_WB_M_24013018_987.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639557/GTX639557_T-45264_20240126_WB_R_24013018_214.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![BRD4 antibody [HL2729] detects BRD4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX639557/GTX639557_T-45264_20240202_ICC_IF_24021917_497.webp "BRD4 antibody [HL2729] detects BRD4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![BRD4 antibody [HL2729] detects BRD4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX639557/GTX639557_T-45264_20240301_IHC-P_24030600_472.webp "BRD4 antibody [HL2729] detects BRD4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639557/GTX639557_45369_20240412_WB_24041523_228.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![BRD4 antibody [HL2729] detects BRD4 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX639557/GTX639557_T-45264_20240619_IHC-P_M_24062501_708.webp "BRD4 antibody [HL2729] detects BRD4 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![BRD4 antibody [HL2729] detects BRD4 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX639557/GTX639557_T-45264_20240711_IHC-P_multiple_R_24071520_180.webp "BRD4 antibody [HL2729] detects BRD4 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. BRD4 stained by BRD4 antibody [HL2729] (GTX639557) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD4 antibody [HL2729] (GTX639557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
BRD4 antibody [HL2729]
GTX639557
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBRD4
Overview
- SupplierGeneTex
- Product NameBRD4 antibody [HL2729]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2729
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID23476
- Target nameBRD4
- Target descriptionbromodomain containing 4
- Target synonymsCAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP, bromodomain-containing protein 4, chromosome-associated protein, female sterile homeotic related gene 4, mitotic chromosome-associated protein
- HostRabbit
- IsotypeIgG
- Protein IDO60885
- Protein NameBromodomain-containing protein 4
- Scientific DescriptionThe protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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