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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA865097LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA865097LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA865097LA01HU at dilution of 1:100

BRD7 Antibody

CSB-PA865097LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBRD7
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Overview

  • Supplier
    Cusabio
  • Product Name
    BRD7 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29117
  • Target name
    BRD7
  • Target description
    bromodomain containing 7
  • Target synonyms
    75 kDa bromodomain protein; BP75; bromodomain-containing protein 7; CELTIX1; NAG4; protein CELTIX-1; SMARCI1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NPI1
  • Protein Name
    Bromodomain-containing protein 7
  • Scientific Description
    Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at Tyr-216. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at Lys-9 (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at Lys-382, and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BRD7 using anti-BRD7 antibody (A01289-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRD7 antigen affinity purified polyclonal antibody (Catalog # A01289-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRD7 at approximately 90KD. The expected band size for BRD7 is at 74KD.
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