![BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160615_IHC-P_2_w_23061202_163.webp)
BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BRG1 antibody [GT2712]
GTX633391
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSMARCA4
Overview
- SupplierGeneTex
- Product NameBRG1 antibody [GT2712]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT2712
- Concentration2.44 mg/ml
- ConjugateUnconjugated
- Gene ID6597
- Target nameSMARCA4
- Target descriptionSWI/SNF related BAF chromatin remodeling complex subunit ATPase 4
- Target synonymsBAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4, ATP-dependent helicase SMARCA4, BRG1-associated factor 190A, BRM/SWI2-related gene 1, SNF2-like 4, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4, brahma protein-like 1, global transcription activator homologous sequence, homeotic gene regulator, mitotic growth and transcription activator, nuclear protein GRB1, protein BRG-1, protein brahma homolog 1, sucrose nonfermenting-like 4, transcription activator BRG1
- HostMouse
- IsotypeIgG1
- Protein IDP51532
- Protein NameSWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
- Scientific DescriptionThe protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160615_IHC-P_w_23061202_117.webp)
![Wild-type (WT) and BRG1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Wild-type (WT) and BRG1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20180216_WB_KO_watermark_w_23061202_386.webp)
![BRG1 antibody [GT2712] detects BRG1 protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: BRG1 stained by BRG1 antibody [GT2712] (GTX633391) diluted at 1:250. Red: SOX2, stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). BRG1 antibody [GT2712] detects BRG1 protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: BRG1 stained by BRG1 antibody [GT2712] (GTX633391) diluted at 1:250. Red: SOX2, stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20171106_IHC-Fr_R_w_23061202_767.webp)
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20161222_WB_M_w_23061202_107.webp)
![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000. Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160505_WB_w_23061202_653.webp)
![BRG1 antibody [GT2712] detects BRG1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRG1 protein stained by BRG1 antibody [GT2712] (GTX633391) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. BRG1 antibody [GT2712] detects BRG1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRG1 protein stained by BRG1 antibody [GT2712] (GTX633391) diluted at 1:100. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160706_IFA_w_23061202_763.webp)
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20161222_WB_R_w_23061202_650.webp)




![IHC-P analysis of human pulmonary adenocarcinoma tissue using GTX04486 BRG1 antibody [MSVA-397R] HistoMAX?. Pulmonary adenocarcinoma with complete loss of BRG1 staining of tumor cells.](https://www.genetex.com/upload/website/prouct_img/normal/GTX04486/GTX04486_20230728_IHC-P_109_23072722_462.webp)
