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Western Blot analysis of 293 cells using BRMS-1 Polyclonal Antibody
Western Blot analysis of 293 cells using BRMS-1 Polyclonal Antibody
Western Blot analysis of 293 cells using BRMS-1 Polyclonal Antibody

BRMS1 Antibody

CSB-PA001048
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBRMS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    BRMS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25855
  • Target name
    BRMS1
  • Target description
    BRMS1 transcriptional repressor and anoikis regulator
  • Target synonyms
    breast cancer metastasis suppressor 1; breast cancer metastasis-suppressor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HCU9
  • Protein Name
    Breast cancer metastasis-suppressor 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BRMS1 using anti-BRMS1 antibody (A03587-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRMS1 antigen affinity purified polyclonal antibody (Catalog # A03587-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRMS1 at approximately 34KD. The expected band size for BRMS1 is at 28KD.
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