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Lane 1: A549 Cells; Lane 2: HL-60 Cells; Lane 3: A431 Cells; Lane 4: Rat Colon; Probed with BUB3 (24D1) Monoclonal Antibody (bsm-54413R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: A549 Cells; Lane 2: HL-60 Cells; Lane 3: A431 Cells; Lane 4: Rat Colon; Probed with BUB3 (24D1) Monoclonal Antibody (bsm-54413R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: A549 Cells; Lane 2: HL-60 Cells; Lane 3: A431 Cells; Lane 4: Rat Colon; Probed with BUB3 (24D1) Monoclonal Antibody (bsm-54413R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

BUB3 Recombinant Antibody

BSM-54413R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBUB3
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Overview

  • Supplier
    Bioss
  • Product Name
    BUB3 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID9184
  • Target name
    BUB3
  • Target description
    BUB3 mitotic checkpoint protein
  • Target synonyms
    BUB3L, hBUB3, mitotic checkpoint protein BUB3, BUB3 budding uninhibited by benzimidazoles 3 homolog, budding uninhibited by benomyl, budding uninhibited by benzimidazoles 3 homolog, mitotic checkpoint component, testicular tissue protein Li 27
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43684
  • Protein Name
    Mitotic checkpoint protein BUB3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of BUB3 using anti-BUB3 antibody (A03118-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB3 antigen affinity purified polyclonal antibody (Catalog # A03118-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BUB3 at approximately 40 kDa. The expected band size for BUB3 is at 37 kDa.
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