Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE GTX113595 diluted at 1:1000
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody [N1C1] (GTX113595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113595/GTX113595_40142_20170901_WB_R_w_23060501_632.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody [N1C1] (GTX113595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 μg of BUB3 antibody [N1C1] (GTX113595). Western blot analysis was performed using BUB3 antibody [N1C1] (GTX113595). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113595/GTX113595_40142_20150618_IP_w_23060501_447.webp "Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 μg of BUB3 antibody [N1C1] (GTX113595). Western blot analysis was performed using BUB3 antibody [N1C1] (GTX113595). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody [N1C1] (GTX113595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113595/GTX113595_40142_20170901_WB_M_w_23060501_363.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody [N1C1] (GTX113595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE GTX113595 diluted at 1:1000
BUB3 antibody [N1C1]
GTX113595
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBUB3
Overview
- SupplierGeneTex
- Product NameBUB3 antibody [N1C1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.32 mg/ml
- ConjugateUnconjugated
- Gene ID9184
- Target nameBUB3
- Target descriptionBUB3 mitotic checkpoint protein
- Target synonymsBUB3L, hBUB3, mitotic checkpoint protein BUB3, BUB3 budding uninhibited by benzimidazoles 3 homolog, budding uninhibited by benomyl, budding uninhibited by benzimidazoles 3 homolog, mitotic checkpoint component, testicular tissue protein Li 27
- HostRabbit
- IsotypeIgG
- Protein IDO43684
- Protein NameMitotic checkpoint protein BUB3
- Scientific DescriptionThis gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8Read this paper
- Chang JF, Lin ST, Hung E, et al. Nuclear proteomics with XRCC3 knockdown to reveal the development of doxorubicin-resistant uterine cancer. Toxicol Sci. 2014,139(2):396-406. doi: 10.1093/toxsci/kfu051Read this paper
Datasheet
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