More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/BUB3 antibody [N1C1]

BUB3 antibody [N1C1]

GTX113595
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetBUB3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    BUB3 antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.32 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9184
  • Target name
    BUB3
  • Target description
    BUB3 mitotic checkpoint protein
  • Target synonyms
    BUB3L, hBUB3, mitotic checkpoint protein BUB3, BUB3 budding uninhibited by benzimidazoles 3 homolog, budding uninhibited by benomyl, budding uninhibited by benzimidazoles 3 homolog, mitotic checkpoint component, testicular tissue protein Li 27
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43684
  • Protein Name
    Mitotic checkpoint protein BUB3
  • Scientific Description
    This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8
    Read this paper
  • Chang JF, Lin ST, Hung E, et al. Nuclear proteomics with XRCC3 knockdown to reveal the development of doxorubicin-resistant uterine cancer. Toxicol Sci. 2014,139(2):396-406. doi: 10.1093/toxsci/kfu051
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Lane 1: A549 Cells; Lane 2: HL-60 Cells; Lane 3: A431 Cells; Lane 4: Rat Colon; Probed with BUB3 (24D1) Monoclonal Antibody (bsm-54413R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Product group Antibodies
Bioss
BUB3 Recombinant AntibodyBSM-54413R
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetBUB3
  • SizePrice
Product group Antibodies
Assay Genie
BUB3 AntibodyCAB6536
TargetBUB3
  • SizePrice
Product group Antibodies
Biomatik
BUB3 Polyclonal AntibodyCAC14581
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetBUB3
  • SizePrice
Figure 1. Western blot analysis of BUB3 using anti-BUB3 antibody (A03118-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB3 antigen affinity purified polyclonal antibody (Catalog # A03118-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BUB3 at approximately 40 kDa. The expected band size for BUB3 is at 37 kDa.
Product group Antibodies
Boster Bio
Anti-BUB3 Antibody Picoband(r)A03118-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetBUB3
  • SizePrice
Product group Antibodies
RayBiotech
Anti-BUB3 Antibody144-06536
ApplicationsImmunoFluorescence, Western Blot
TargetBUB3
  • SizePrice
Anti-BUB3 Antibody
Product group Antibodies
Antibodies.com
Anti-BUB3 AntibodyA98261
ApplicationsWestern Blot, ELISA
  • SizePrice
ICC/IF analysis of Jurkat cells using GTX31314 BUB3 antibody. Working concentration : 10 microg/ml
Product group Antibodies
GeneTex
BUB3 antibodyGTX31314
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetBUB3
  • SizePrice
WB analysis of various sample lysates using GTX33047 BUB3 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
Product group Antibodies
GeneTex
BUB3 antibodyGTX33047
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetBUB3
  • SizePrice
WB analysis of extracts from U87 (A) and U87 EGFRvIII (B) cells in mitosis using GTX55298 BUB3 (phospho Tyr207) antibody (PMID: 24316223).
Product group Antibodies
GeneTex
BUB3 (phospho Tyr207) antibodyGTX55298
ApplicationsWestern Blot
TargetBUB3
  • SizePrice
WB analysis of HeLa whole cell lysate using GTX57601 BUB3 antibody. Loading : 40 microg Dilution : 1:1000
Product group Antibodies
GeneTex
BUB3 antibody [AT2H6]GTX57601
ApplicationsWestern Blot
TargetBUB3
  • SizePrice