c-Kit / CD117 Antibody (aa100-300, clone KIT/983)

LifeSpan BioSciences

c-Kit / CD117 Antibody (aa100-300, clone KIT/983)

23

Human C-Kit / CD117

This mAb recognizes a protein of 145kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117/c-Kit has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposis sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117/c-Kit is also useful in recognizing myeloblasts in bone marrow biopsy and clot section. Optimal dilution of the c-Kit antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flo (0.5 - 1 µg/10E6 cells), IF (0.5 - 1 µg/ml), IHC, IHC-P (2 - 4 µg/ml), WB (2 - 5 µg/ml) This mAb recognizes a protein of 145kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117/c-Kit has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposis sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117/c-Kit is also useful in recognizing myeloblasts in bone marrow biopsy and clot section. Optimal dilution of the c-Kit antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min

Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

KIT/983

0.2 mg/ml

Unconjugated

...

KIT

KIT proto-oncogene, receptor tyrosine kinase

CD117; C-Kit; c-Kit protooncogene; mast/stem cell growth factor receptor Kit; MASTC; p145 c-kit; PBT; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Mouse

IgG1

Human

2°C to 8°C

12352203