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ICC/IF analysis of methanol-fixed HeLa cells using GTX50099 c-Rel (phospho Ser503) antibody.
ICC/IF analysis of methanol-fixed HeLa cells using GTX50099 c-Rel (phospho Ser503) antibody.
ICC/IF analysis of methanol-fixed HeLa cells using GTX50099 c-Rel (phospho Ser503) antibody.

c-Rel (phospho Ser503) antibody

GTX50099
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetREL
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Overview

  • Supplier
    GeneTex
  • Product Name
    c-Rel (phospho Ser503) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:100-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5966
  • Target name
    REL
  • Target description
    REL proto-oncogene, NF-kB subunit
  • Target synonyms
    C-Rel; HIVEN86A; oncogene REL, avian reticuloendotheliosis; proto-oncogene c-Rel; v-rel avian reticuloendotheliosis viral oncogene homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04864
  • Protein Name
    Proto-oncogene c-Rel
  • Scientific Description
    This gene encodes a protein that belongs to the Rel homology domain/immunoglobulin-like fold, plexin, transcription factor (RHD/IPT) family. Members of this family regulate genes involved in apoptosis, inflammation, the immune response, and oncogenic processes. This proto-oncogene plays a role in the survival and proliferation of B lymphocytes. Mutation or amplification of this gene is associated with B-cell lymphomas, including Hodgkins lymphoma. Single nucleotide polymorphisms in this gene are associated with susceptibility to ulcerative colitis and rheumatoid arthritis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of c-Rel/REL using anti-c-Rel/REL antibody (A01880-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Rel/REL antigen affinity purified polyclonal antibody (Catalog # A01880-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Rel/REL at approximately 78 kDa. The expected band size for c-Rel/REL is at 69 kDa.
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