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ProductsBack/c-Src Kinase / CSK Antibody

c-Src Kinase / CSK Antibody

LS-C812554
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetCSK
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    c-Src Kinase / CSK Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), IF (1:200 - 1:1000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1445
  • Target name
    CSK
  • Target description
    C-terminal Src kinase
  • Target synonyms
    CSK, non-receptor tyrosine kinase; C-Src kinase; c-src tyrosine kinase; protein-tyrosine kinase CYL; tyrosine-protein kinase CSK
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CSK using anti-CSK antibody (PB9102). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CSK protein 0.5 ng After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSK antigen affinity purified polyclonal antibody (Catalog # PB9102) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSK at approximately 47 kDa. The expected band size for CSK is at 47 kDa.
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