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CSK Monoclonal Antibody

CAB0666
Assay Genie
TargetCSK
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    CSK Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1445
  • Target name
    CSK
  • Target description
    C-terminal Src kinase
  • Target synonyms
    tyrosine-protein kinase CSK, C-Src kinase, CSK, non-receptor tyrosine kinase, c-src tyrosine kinase, protein-tyrosine kinase CYL
  • Host
    Rabbit
  • Protein IDP41240
  • Protein Name
    Tyrosine-protein kinase CSK
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CSK using anti-CSK antibody (PB9102). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CSK protein 0.5 ng After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSK antigen affinity purified polyclonal antibody (Catalog # PB9102) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSK at approximately 47 kDa. The expected band size for CSK is at 47 kDa.
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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
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