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Western Blot analysis of 22RV1 cells using Csk Polyclonal Antibody
Western Blot analysis of 22RV1 cells using Csk Polyclonal Antibody
Western Blot analysis of 22RV1 cells using Csk Polyclonal Antibody

CSK Antibody

CSB-PA001798
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCSK
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Overview

  • Supplier
    Cusabio
  • Product Name
    CSK Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1445
  • Target name
    CSK
  • Target description
    C-terminal Src kinase
  • Target synonyms
    CSK, non-receptor tyrosine kinase; C-Src kinase; c-src tyrosine kinase; protein-tyrosine kinase CYL; tyrosine-protein kinase CSK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41240
  • Protein Name
    Tyrosine-protein kinase CSK
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CSK using anti-CSK antibody (PB9102). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human CSK protein 0.5 ng After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSK antigen affinity purified polyclonal antibody (Catalog # PB9102) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSK at approximately 47 kDa. The expected band size for CSK is at 47 kDa.
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