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ProductsBack/C14orf151 (INF2) Rabbit Polyclonal Antibody
WB Suggested Anti-INF2 Antibody Titration: 1.0 ug/ml Positive Control: HepG2 Whole CellINF2 is supported by BioGPS gene expression data to be expressed in HepG2
WB Suggested Anti-INF2 Antibody Titration: 1.0 ug/ml Positive Control: HepG2 Whole CellINF2 is supported by BioGPS gene expression data to be expressed in HepG2
WB Suggested Anti-INF2 Antibody Titration: 1.0 ug/ml Positive Control: HepG2 Whole CellINF2 is supported by BioGPS gene expression data to be expressed in HepG2

C14orf151 (INF2) Rabbit Polyclonal Antibody

TA358506
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetINF2
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    C14orf151 (INF2) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID64423
  • Target name
    INF2
  • Target description
    inverted formin 2
  • Target synonyms
    C14orf151; C14orf173; CMTDIE; FSGS5; HBEAG-binding protein 2 binding protein C; HBEBP2-binding protein C; inverted formin, FH2 and WH2 domain containing; inverted formin-2; pp9484
  • Host
    Rabbit
  • Protein IDQ27J81
  • Protein Name
    Inverted formin-2
  • Scientific Description
    INF2 Antibody - C-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of INF2 using anti-INF2 antibody (A02710-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INF2 antigen affinity purified polyclonal antibody (Catalog # A02710-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INF2 at approximately 180 kDa. The expected band size for INF2 is at 136 kDa.
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