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INF2 antibody

GTX130714
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
DownloadDatasheet
Product group Antibodies
TargetINF2
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Overview

  • Supplier
    GeneTex
  • Product Name
    INF2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.58 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64423
  • Target name
    INF2
  • Target description
    inverted formin 2
  • Target synonyms
    C14orf151; C14orf173; CMTDIE; FSGS5; HBEAG-binding protein 2 binding protein C; HBEBP2-binding protein C; inverted formin, FH2 and WH2 domain containing; inverted formin-2; pp9484
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ27J81
  • Protein Name
    Inverted formin-2
  • Scientific Description
    This gene represents a member of the formin family of proteins. It is considered a diaphanous formin due to the presence of a diaphanous inhibitory domain located at the N-terminus of the encoded protein. Studies of a similar mouse protein indicate that the protein encoded by this locus may function in polymerization and depolymerization of actin filaments. Mutations at this locus have been associated with focal segmental glomerulosclerosis 5.[provided by RefSeq, Aug 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of INF2 using anti-INF2 antibody (A02710-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INF2 antigen affinity purified polyclonal antibody (Catalog # A02710-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INF2 at approximately 180 kDa. The expected band size for INF2 is at 136 kDa.
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