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ProductsBack/C2orf30 antibody, Internal
WB analysis of THP-1 cells using GTX45297 C2orf30 antibody at 0.2-1microg/ml.
WB analysis of THP-1 cells using GTX45297 C2orf30 antibody at 0.2-1microg/ml.
WB analysis of THP-1 cells using GTX45297 C2orf30 antibody at 0.2-1microg/ml.

C2orf30 antibody, Internal

GTX45297
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetERLEC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    C2orf30 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27248
  • Target name
    ERLEC1
  • Target description
    endoplasmic reticulum lectin 1
  • Target synonyms
    C2orf30, CIM, CL24936, CL25084, HEL117, XTP3-B, XTP3TPB, endoplasmic reticulum lectin 1, ER lectin, XTP3-transactivated gene B protein, XTP3-transactivated protein B, cancer invasion and metastasis-related, epididymis luminal protein 117, erlectin 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DZ1
  • Protein Name
    Endoplasmic reticulum lectin 1
  • Scientific Description
    This gene encodes a resident endoplasmic reticulum (ER) protein that functions in N-glycan recognition. This protein is thought to be involved in ER-associated degradation via its interaction with the membrane-associated ubiquitin ligase complex. It also functions as a regulator of multiple cellular stress-response pathways in a manner that promotes metastatic cell survival. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 21. [provided by RefSeq, Aug 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERLEC1 using anti-ERLEC1 antibody (A11245-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERLEC1 antigen affinity purified polyclonal antibody (Catalog # A11245-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERLEC1 at approximately 55 kDa. The expected band size for ERLEC1 is at 55 kDa.
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