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ERLEC1 Antibody (aa359-408)

LS-C116609
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetERLEC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ERLEC1 Antibody (aa359-408)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.2 - 1 µg/ml) Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID27248
  • Target name
    ERLEC1
  • Target description
    endoplasmic reticulum lectin 1
  • Target synonyms
    C2orf30, CIM, CL24936, CL25084, HEL117, XTP3-B, XTP3TPB, endoplasmic reticulum lectin 1, ER lectin, XTP3-transactivated gene B protein, XTP3-transactivated protein B, cancer invasion and metastasis-related, epididymis luminal protein 117, erlectin 1
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERLEC1 using anti-ERLEC1 antibody (A11245-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERLEC1 antigen affinity purified polyclonal antibody (Catalog # A11245-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERLEC1 at approximately 55 kDa. The expected band size for ERLEC1 is at 55 kDa.
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