C3 / C3b antibody [C3], C-term
GTX101316
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
TargetC3
Overview
- SupplierGeneTex
- Product NameC3 / C3b antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.08 mg/ml
- ConjugateUnconjugated
- Gene ID718
- Target nameC3
- Target descriptioncomplement C3
- Target synonymsacylation-stimulating protein cleavage product; AHUS5; ARMD9; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a; C3a anaphylatoxin; C3b; complement C3; complement component 3; complement component C3a; complement component C3b; CPAMD1; epididymis secretory sperm binding protein Li 62p; HEL-S-62p; prepro-C3
- HostRabbit
- IsotypeIgG
- Protein IDP01024
- Protein NameComplement C3
- Scientific DescriptionComplement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Exposure to fluoride aggravates the impairment in learning and memory and neuropathological lesions in mice carrying the APP/PS1 double-transgenic mutation. Cao K et al., 2019 Apr 22, Alzheimers Res TherRead more
- Modulation of Iberian Ribbed Newt Complement Component C3 by Stressors Similar to those Encountered during a Stay Onboard the International Space Station. Gueguinou N et al., 2019 Mar 29, Int J Mol SciRead more
- Human skin mast cells express complement factors C3 and C5. Fukuoka Y et al., 2013 Aug 15, J ImmunolRead more
- Obesity-associated autoantibody production requires AIM to retain the immunoglobulin M immune complex on follicular dendritic cells. Arai S et al., 2013 Apr 25, Cell RepRead more
- Improved capillary electrophoresis method for the analysis of carbohydrate-deficient transferrin in human serum, avoiding interference by complement C3. Kuroda Y et al., 2013 Mar 25, J Pharm Biomed AnalRead more

![The two groups of complement C3 proteins can be purified by GTX02807 C3 antibody [M68] immunoaffinity chromatography from the partially purified milk fractions. Human milk proteins were loaded onto CM-Sepharose 4B column and proteins, including C3, were eluted by different salt (NaCl) concentration. The slat concentration was smaller than 0.3 N. The CM low salt fractions were collected and loaded to the mAb M68-Sepharose column to purify C3. After washing the immunoaffinity column, the captured proteins were eluted by 0.1 N glycine buffer pH 2.4. The eluates were collected into several fractions, E1, E2, E3, E4, E5 and E6. E1-E4 fractions were analyzed by SDS-PAGE and the eluted proteins were stained by CBB. The protein band 2 and 7 as indicated are sliced out and subjected to MS/MS protein identification (by Prottech Inc.), confirming these bands to be complement C3. The relative abundance of peptides matching to C3 is 98% for band 2 and 96% for band 7. For the best detection sensitivity, the samples should be treated under non-boiled and non-reducing conditions. Lane A : Input (partially purified milk fractions) Lane B : Flow through Lane C : Elution 1 Lane D : Elution 2 Lane E : Elution 3 Lane F : Elution 4 Loading : 20 microl](https://www.genetex.com/upload/website/prouct_img/normal/GTX02807/GTX02807_20201130_IP_w_23053122_193.webp)