C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term (GTX101316) diluted at 1:200. Blue: Hoechst 33342 staining.
![C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. C3 antibody [C3], C-term (GTX101316) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101316/GTX101316_42116_20151005_IHC-P_M_w_23060100_661.webp "C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. C3 antibody [C3], C-term (GTX101316) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg of C3 antibody [C3], C-term (GTX101316). Western blot analysis was performed using C3 antibody [C3], C-term (GTX101316). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101316/GTX101316_41710_20150209_IP_w_23060100_932.webp "Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg of C3 antibody [C3], C-term (GTX101316). Western blot analysis was performed using C3 antibody [C3], C-term (GTX101316). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 / C3b antibody [C3], C-term (GTX101316) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101316/GTX101316_44818_20221014_WB_plasma_25061003_462.webp "Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 / C3b antibody [C3], C-term (GTX101316) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term (GTX101316) diluted at 1:200. Blue: Hoechst 33342 staining.
C3 / C3b antibody [C3], C-term
GTX101316
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman, Mouse
TargetC3
Overview
- SupplierGeneTex
- Product NameC3 / C3b antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.07 mg/ml
- ConjugateUnconjugated
- Gene ID718
- Target nameC3
- Target descriptioncomplement C3
- Target synonymsAHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p, complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, acylation-stimulating protein cleavage product, complement component 3, complement component C3a, complement component C3b, epididymis secretory sperm binding protein Li 62p, prepro-C3
- HostRabbit
- IsotypeIgG
- Protein IDP01024
- Protein NameComplement C3
- Scientific DescriptionComplement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![The two groups of complement C3 proteins can be purified by GTX02807 C3 antibody [M68] immunoaffinity chromatography from the partially purified milk fractions. Human milk proteins were loaded onto CM-Sepharose 4B column and proteins, including C3, were eluted by different salt (NaCl) concentration. The slat concentration was smaller than 0.3 N. The CM low salt fractions were collected and loaded to the mAb M68-Sepharose column to purify C3. After washing the immunoaffinity column, the captured proteins were eluted by 0.1 N glycine buffer pH 2.4. The eluates were collected into several fractions, E1, E2, E3, E4, E5 and E6. E1-E4 fractions were analyzed by SDS-PAGE and the eluted proteins were stained by CBB. The protein band 2 and 7 as indicated are sliced out and subjected to MS/MS protein identification (by Prottech Inc.), confirming these bands to be complement C3. The relative abundance of peptides matching to C3 is 98% for band 2 and 96% for band 7. For the best detection sensitivity, the samples should be treated under non-boiled and non-reducing conditions. Lane A : Input (partially purified milk fractions) Lane B : Flow through Lane C : Elution 1 Lane D : Elution 2 Lane E : Elution 3 Lane F : Elution 4 Loading : 20 μl](https://www.genetex.com/upload/website/prouct_img/normal/GTX02807/GTX02807_20201130_IP_w_23053122_193.webp)
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