C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term (GTX101316) diluted at 1:200. Blue: Hoechst 33342 staining.
![C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. C3 antibody [C3], C-term (GTX101316) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101316/GTX101316_42116_20151005_IHC-P_M_w_23060100_661.webp "C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. C3 antibody [C3], C-term (GTX101316) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg of C3 antibody [C3], C-term (GTX101316). Western blot analysis was performed using C3 antibody [C3], C-term (GTX101316). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101316/GTX101316_41710_20150209_IP_w_23060100_932.webp "Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg of C3 antibody [C3], C-term (GTX101316). Western blot analysis was performed using C3 antibody [C3], C-term (GTX101316). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 / C3b antibody [C3], C-term (GTX101316) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101316/GTX101316_44818_20221014_WB_plasma_25061003_462.webp "Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C3 / C3b antibody [C3], C-term (GTX101316) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term (GTX101316) diluted at 1:200. Blue: Hoechst 33342 staining.
C3 / C3b antibody [C3], C-term
GTX101316
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman, Mouse
TargetC3
Overview
- SupplierGeneTex
- Product NameC3 / C3b antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.08 mg/ml
- ConjugateUnconjugated
- Gene ID718
- Target nameC3
- Target descriptioncomplement C3
- Target synonymsAHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p, complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, acylation-stimulating protein cleavage product, complement component 3, complement component C3a, complement component C3b, epididymis secretory sperm binding protein Li 62p, prepro-C3
- HostRabbit
- IsotypeIgG
- Protein IDP01024
- Protein NameComplement C3
- Scientific DescriptionComplement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Cao K, Xiang J, Dong YT, et al. Exposure to fluoride aggravates the impairment in learning and memory and neuropathological lesions in mice carrying the APP/PS1 double-transgenic mutation. Alzheimers Res Ther. 2019,11(1):35. doi: 10.1186/s13195-019-0490-3Read this paper
- Guéguinou N, Jeandel J, Kaminski S, et al. Modulation of Iberian Ribbed Newt Complement Component C3 by Stressors Similar to those Encountered during a Stay Onboard the International Space Station. Int J Mol Sci. 2019,20(7). doi: 10.3390/ijms20071579Read this paper
- Fukuoka Y, Hite MR, Dellinger AL, et al. Human skin mast cells express complement factors C3 and C5. J Immunol. 2013,191(4):1827-34. doi: 10.4049/jimmunol.1202889Read this paper
- Arai S, Maehara N, Iwamura Y, et al. Obesity-associated autoantibody production requires AIM to retain the immunoglobulin M immune complex on follicular dendritic cells. Cell Rep. 2013,3(4):1187-98. doi: 10.1016/j.celrep.2013.03.006Read this paper
- Kuroda Y, Hamaguchi R, Moriyama K, et al. Improved capillary electrophoresis method for the analysis of carbohydrate-deficient transferrin in human serum, avoiding interference by complement C3. J Pharm Biomed Anal. 2013,76:81-6. doi: 10.1016/j.jpba.2012.12.011Read this paper
Datasheet
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