![Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody [HL2744] (GTX639573) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody [HL2744] (GTX639573) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639573/GTX639573_T-45271_20240712_WB_B_24071520_907.webp)
Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody [HL2744] (GTX639573) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
C3a Receptor antibody [HL2744]
GTX639573
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetC3AR1
Overview
- SupplierGeneTex
- Product NameC3a Receptor antibody [HL2744]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2744
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID719
- Target nameC3AR1
- Target descriptioncomplement C3a receptor 1
- Target synonymsAZ3B, C3AR, HNFAG09, C3a anaphylatoxin chemotactic receptor, complement component 3 receptor 1, complement component 3a receptor 1
- HostRabbit
- IsotypeIgG
- Protein IDQ16581
- Protein NameC3a anaphylatoxin chemotactic receptor
- Scientific DescriptionC3a is an anaphylatoxin released during activation of the complement system. The protein encoded by this gene is an orphan G protein-coupled receptor for C3a. Binding of C3a by the encoded receptor activates chemotaxis, granule enzyme release, superoxide anion production, and bacterial opsonization. [provided by RefSeq, May 2016]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![C3a Receptor antibody [HL2744] detects C3a Receptor protein by immunofluorescent analysis. Sample: HL-60 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3a Receptor stained by C3a Receptor antibody [HL2744] (GTX639573) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). C3a Receptor antibody [HL2744] detects C3a Receptor protein by immunofluorescent analysis. Sample: HL-60 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3a Receptor stained by C3a Receptor antibody [HL2744] (GTX639573) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX639573/GTX639573_T-45271_20240705_ICC_IF_24081300_592.webp)
![C3a Receptor antibody [HL2744] (GTX639573) detects C3a Receptor protein by flow cytometry analysis. Sample: non-fixed and non-permeabilized HL-60 or HeLa cells were stained with 5μg/ml GTX639573 (red) or a Rabbit monoclonal IgG isotype control (gray) in 1% FBS/ 5mM EDTA/PBS at 4oC for 1 hour. C3a Receptor antibody [HL2744] (GTX639573) detects C3a Receptor protein by flow cytometry analysis. Sample: non-fixed and non-permeabilized HL-60 or HeLa cells were stained with 5μg/ml GTX639573 (red) or a Rabbit monoclonal IgG isotype control (gray) in 1% FBS/ 5mM EDTA/PBS at 4oC for 1 hour.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639573/GTX639573_T-45271_20250214_FACS_25021923_653.webp)




![C3a Receptor antibody [HL2831] detects C3a Receptor protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: C3a Receptor stained by C3a Receptor antibody [HL2831] (GTX640102) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX640102/GTX640102_T-45355_20240426_ICC_IF_B_24051400_888.webp)
![C3a Receptor antibody [HL2855] detects C3a Receptor protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: C3a Receptor stained by C3a Receptor antibody [HL2855] (GTX640126) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX640126/GTX640126_T-45355_20240426_ICC_IF_B_24051400_806.webp)
