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293T cell lysates probed with C9orf72 Polyclonal Antibody, Unconjugated (bs-8595R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
293T cell lysates probed with C9orf72 Polyclonal Antibody, Unconjugated (bs-8595R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
293T cell lysates probed with C9orf72 Polyclonal Antibody, Unconjugated (bs-8595R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

C9orf72 Polyclonal Antibody

BS-8595R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetC9orf72
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Overview

  • Supplier
    Bioss
  • Product Name
    C9orf72 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID203228
  • Target name
    C9orf72
  • Target description
    C9orf72-SMCR8 complex subunit
  • Target synonyms
    ALSFTD, DENND9, DENNL72, FTDALS, FTDALS1, guanine nucleotide exchange factor C9orf72, guanine nucleotide exchange C9orf72, protein C9orf72
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96LT7
  • Protein Name
    Guanine nucleotide exchange factor C9orf72
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of C9ORF72 using anti-C9ORF72 antibody (A00419-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human Hela whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C9ORF72 antigen affinity purified polyclonal antibody (Catalog # A00419-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C9ORF72 at approximately 65KD. The expected band size for C9ORF72 is at 65KD.
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