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ProductsBack/CA II Recombinant Antibody, Biotin Conjugated

CA II Recombinant Antibody, Biotin Conjugated

BSM-61561R-BIOTIN
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCA2
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Overview

  • Supplier
    Bioss
  • Product Name
    CA II Recombinant Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Biotin
  • Gene ID760
  • Target name
    CA2
  • Target description
    carbonic anhydrase 2
  • Target synonyms
    CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282, carbonic anhydrase 2, carbonate dehydratase II, carbonic anhydrase B, carbonic anhydrase C, carbonic anhydrase II, carbonic dehydratase, cyanamide hydratase CA2, epididymis luminal protein 76, epididymis secretory protein Li 282
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00918
  • Protein Name
    Carbonic anhydrase 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CA2 using anti-CA2 antibody (PB9989). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA2 antigen affinity purified polyclonal antibody (Catalog # PB9989) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA2 at approximately 29 kDa. The expected band size for CA2 is at 29 kDa.
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