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Western Blot Positive WB detected in: Rat kidney tissue, Rat heart tissue, Rat liver tissue, Mouse lung tissue, Mouse kidney tissue All lanes: CA2 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
Western Blot Positive WB detected in: Rat kidney tissue, Rat heart tissue, Rat liver tissue, Mouse lung tissue, Mouse kidney tissue All lanes: CA2 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
Western Blot Positive WB detected in: Rat kidney tissue, Rat heart tissue, Rat liver tissue, Mouse lung tissue, Mouse kidney tissue All lanes: CA2 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa

CA2 Antibody

CSB-PA004370HA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCA2
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Overview

  • Supplier
    Cusabio
  • Product Name
    CA2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID760
  • Target name
    CA2
  • Target description
    carbonic anhydrase 2
  • Target synonyms
    CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282, carbonic anhydrase 2, carbonate dehydratase II, carbonic anhydrase B, carbonic anhydrase C, carbonic anhydrase II, carbonic dehydratase, cyanamide hydratase CA2, epididymis luminal protein 76, epididymis secretory protein Li 282
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00918
  • Protein Name
    Carbonic anhydrase 2
  • Scientific Description
    Essential for bone resorption and osteoclast differentiation .Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CA2 using anti-CA2 antibody (PB9989). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA2 antigen affinity purified polyclonal antibody (Catalog # PB9989) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA2 at approximately 29 kDa. The expected band size for CA2 is at 29 kDa.
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