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ProductsBack/CA2 / Carbonic Anhydrase II Antibody (Lyophilized)

CA2 / Carbonic Anhydrase II Antibody (Lyophilized)

LS-C209639
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCA2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CA2 / Carbonic Anhydrase II Antibody (Lyophilized)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Anti CARBONIC ANHYDRASE II Antibody has been assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMBE as a substrate for 30 minutes at room temperature.. ELISA (1:20000 - 1:100000), IHC, WB (1:2000 - 1:10000) Anti CARBONIC ANHYDRASE II Antibody has been assayed against 1.0 ug of Carbonic Anhydrase II [Bovine Erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMBE as a substrate for 30 minutes at room temperature.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID760
  • Target name
    CA2
  • Target description
    carbonic anhydrase 2
  • Target synonyms
    CAC; CAII; CA-II; Car2; carbonate dehydratase II; carbonic anhydrase 2; carbonic anhydrase B; carbonic anhydrase C; carbonic anhydrase II; carbonic dehydratase; epididymis luminal protein 76; epididymis secretory protein Li 282; HEL-76; HEL-S-282
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CA2 using anti-CA2 antibody (PB9989). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA2 antigen affinity purified polyclonal antibody (Catalog # PB9989) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA2 at approximately 29 kDa. The expected band size for CA2 is at 29 kDa.
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