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CAD Antibody (YA2523, Rabbit)

HY-P82778
MedChem Express
TargetCAD
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    CAD Antibody (YA2523, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID790
  • Target name
    CAD
  • Target description
    carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
  • Target synonyms
    CAD protein; CAD trifunctional protein; CDG1Z; DEE50; EIEE50; GATD4; multifunctional protein CAD
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    CAD Antibody (YA2523) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2523), targeting CAD, with a predicted molecular weight of 243 kDa (observed band size: 260 kDa). CAD Antibody (YA2523) can be used for WB, ICC/IF, IP, FC experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CAD using anti-CAD antibody (A00463-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAD antigen affinity purified polyclonal antibody (Catalog # A00463-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAD at approximately 270KD. The expected band size for CAD is at 243KD.
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