Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638641/GTX638641_T-45068_20231013_WB_M_R_23101921_686.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638641/GTX638641_T-45068_20231013_WB_M_tissue_23101921_217.webp "Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638641/GTX638641_T-45068_20231013_WB_testis_23101921_675.webp "Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638641/GTX638641_T-45068_20231013_WB_B_23101921_537.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638641/GTX638641_45257_20231215_WB_23121922_877.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody [HL2419] (GTX638641) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Calcineurin B antibody [HL2419]
GTX638641
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPPP3R1
Overview
- SupplierGeneTex
- Product NameCalcineurin B antibody [HL2419]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2419
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5534
- Target namePPP3R1
- Target descriptionprotein phosphatase 3 regulatory subunit B, alpha
- Target synonymsCALNB1, CNB, CNB1, calcineurin subunit B type 1, calcineurin B, type I (19kDa), protein phosphatase 2B regulatory subunit 1, protein phosphatase 2B regulatory subunit B alpha
- HostRabbit
- IsotypeIgG
- Protein IDP63098
- Protein NameCalcineurin subunit B type 1
- Scientific DescriptionEnables cyclosporin A binding activity; phosphatase binding activity; and protein domain specific binding activity. Involved in calcineurin-NFAT signaling cascade and positive regulation of transcription by RNA polymerase II. Part of calcineurin complex. Implicated in Alzheimers disease and dilated cardiomyopathy. [provided by Alliance of Genome Resources, Apr 2022]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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