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ProductsBack/Calcium Sensing Receptor (CASR) Rabbit Polyclonal Antibody
Western blot analyzes of CaSR antibody (AP20293PU-N) in extracts from LOVO cells.
Western blot analyzes of CaSR antibody (AP20293PU-N) in extracts from LOVO cells.
Western blot analyzes of CaSR antibody (AP20293PU-N) in extracts from LOVO cells.

Calcium Sensing Receptor (CASR) Rabbit Polyclonal Antibody

AP20293PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCASR
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Calcium Sensing Receptor (CASR) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID846
  • Target name
    CASR
  • Target description
    calcium sensing receptor
  • Target synonyms
    CAR, EIG8, FHH, FIH, GPRC2A, HHC, HHC1, HYPOC1, NSHPT, PCAR1, hCasR, extracellular calcium-sensing receptor, parathyroid Ca(2+)-sensing receptor 1, parathyroid cell calcium-sensing receptor 1
  • Host
    Rabbit
  • Protein IDP41180
  • Protein Name
    Extracellular calcium-sensing receptor
  • Scientific Description
    Calcium Sensing Receptor (CASR) rabbit polyclonal antibody, Aff - Purified
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CASR using anti-CASR antibody (PB9924). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASR antigen affinity purified polyclonal antibody (Catalog # PB9924) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASR at approximately 150 kDa. The expected band size for CASR is at 121 kDa.
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