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Western blot All lanes: CALCOCO2 antibody at 3.36ug/ml Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 48, 55, 56, 44 kDa Observed band size: 56 kDa
Western blot All lanes: CALCOCO2 antibody at 3.36ug/ml Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 48, 55, 56, 44 kDa Observed band size: 56 kDa
Western blot All lanes: CALCOCO2 antibody at 3.36ug/ml Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 48, 55, 56, 44 kDa Observed band size: 56 kDa

CALCOCO2 Antibody

CSB-PA614394ESR1HU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCALCOCO2
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Overview

  • Supplier
    Cusabio
  • Product Name
    CALCOCO2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10241
  • Target name
    CALCOCO2
  • Target description
    calcium binding and coiled-coil domain 2
  • Target synonyms
    antigen nuclear dot 52 kDa protein; calcium-binding and coiled-coil domain-containing protein 2; NDP52; nuclear domain 10 protein 52; nuclear domain 10 protein NDP52; nuclear dot protein 52
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13137
  • Protein Name
    Calcium-binding and coiled-coil domain-containing protein 2
  • Scientific Description
    Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting galectin-8-associated bacteria for autophagy (PubMed:22246324). Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation (PubMed:23022382, PubMed:25771791). Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C (PubMed:23022382, PubMed:25771791). May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion (PubMed:17635994).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDP52/CALCOCO2 using anti-NDP52/CALCOCO2 antibody (A05876-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human U20S wholel cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDP52/CALCOCO2 antigen affinity purified polyclonal antibody (Catalog # A05876-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDP52/CALCOCO2 at approximately 52KD. The expected band size for NDP52/CALCOCO2 is at 52KD.
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